Analysis of Ethanol in Moonshine

Analysis of fermentation alcohol in Moonshine Using the GC-MS Jennifer Greene &8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212- 4 whitethorn 2012 &8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212&8212- 4 May 2012 Introduction In this experiment splosh chromatography is applied to separate the water- ethyl alcohol mixing. This method acting is often used in the determination of alcohol in caudex or urine. One obvious application is when law enforcement agencies need to curb whether or non some whizz is inebriated. In these cases, high sensitivity is demand since 0. 1% blood alcohol content is considered to be legally excite in most adduces.Our own determination lead deal with higher(prenominal) concentrations (up to 25% by volume) which are more typical of alcohol levels nominate in many alcoholic beverages. Chromatography is a physical method of judgment of conviction interval in which the components to be separa ted are distributed between two var.s, one of the phases constituting a nonmoving arse of large surface area, the other world a fluid that percolates finished or along the stationary bed Gas chromatography (GC) is a powerful and widely used tool for the insulation, recognition and quantitation of components in a mixture.In this technique, a type is converted to the vapor state and a flowing stream of carrier gas (often helium or nitrogen) sweeps the sample into a thermally-controlled column. In the case of gas-liquid chromatography, the column is usually packed with hearty particles that are coated with a non-volatile liquid, referred to as the stationary phase. As the sample mixture moves through the column, sample components that interact strongly with the stationary phase authorise more time in the stationary phase vs. the moving gas phase and thus require more time to move through the column.The goal of GC is to separate and detect components of a mixture as efficient ly as possible and it is thus delectable to have a measure of the efficiency of a given chromatographic column. One common way to express column efficiency is by calculating the height equivalent to a theoretical plate (HETP) which is the space of the column divided by the number of theoretical plates. HETP = l/n The number of theoretical plates (n) of a column with respect to a finicky compound can be found using the following equivalence N = 16(tr/w)2 = 5. 55(tr/w1/2)2Where w is the peak width measured in the equivalent units as tr and w1/2 is the peak width measured at half(a) of the peak height. Materials GC-MS with database Vials with lids Cherry moonshine Disposable pipettes Ethanol 100% map 1. Set up procedure to Set Detector A On (Thermal Conductivity Detector) Packed Column Head Pressure 30 psi Column Carbowax 20M, 1/8&8243 OD, length = 6 feet Oven Temperature 110C Detector Temperature 150C Injection Temperature 150C 2. With the fluid pipette fill separate vials wi th the cherry moonshine and pure ethanol. . go bad the procedure then define the ethanol peak with three redundant peaks. This is the chromatograph for Cherry Moonshine. This is the chromatograph for Cherry Moonshine. Data The saturnine arrows are pointing to the ethanol peak. The blue arrows are pointing to the ethanol peak. Conclusion Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of the phases constituting a stationary bed of large surface area, the other cosmos a fluid that percolates through or along the stationary bed.Gas chromatography (GC) is a powerful and widely used tool for the separation, identification and quantitation of components in a mixture. In this technique, a sample is converted to the vapor state and a flowing stream of carrier gas (often helium or nitrogen) sweeps the sample into a thermally-controlled column. In the case of gas-liquid chromatography, the column is usually packed with solid particles that are coated with a non-volatile liquid, referred to as the stationary phase.As the sample mixture moves through the column, sample components that interact strongly with the stationary phase spend more time in the stationary phase vs. the moving gas phase and thus require more time to move through the column. This experiment demonstrated how the analysis of an unknown substance can be determined from the separate peaks. If you select an individual peak on the chromatograph, the database will bring up possible matches of the compound.You would need to look at the chain reactor spectrograph of that peak to see if it matches the compound of interest. If it does, then you have confirm one compound that is present in the substance. If it is not a match, inhabit to search the database until you confirm the peaks identity. From this experiment, the peak for ethanol was not determined using the mass spectrograph of the gas chromatography however, we ran pur e ethanol on the GC-MS and compared the two chromatographs to determine the ethanol peak.