Human Gastric Adenocarcinoma Cell Line Essay -- Chemistry, Cell

IntroductionThere atomic number 18 many variant cellular phone runs that collapse been used in order to examine genotoxicity motion-picture show of agents such as human gastric adenocarcinoma cell line - TMK1, human colon cancer - COL2, particularly human B lymphoblastoid cell line - TK6 is more often used. TK6 is a cell line heterozygous at the thymidine kinase locus from a patient with chronic myelogenous leukemia (CML) in T cell lineage blast crisis which included Philadelphia chromosome t(922)(q34q11), an abnormality in chromosome 6 (a deletion of the long arm), and chromosome 7, for example, del(6)(q21) ins(1-)(q21-) del(1)(q21q32) dic(7)(p13 cen q3211.2 cen pter) (Watanabe et al., 1995 Tomita et al., 1998). These cells are relatively stable p53-normal immortal, grant low mutation frequencies in gene and chromosome, and are proficient in mismatch repair (Tomita-Mitchel et al., 2000), from these properties TK6 cell line is applied in this orbit for assessing toxicity of a nti-cancer agents. Polyynes containing triple bonds which are presented in many natural sources, have been considered in medicine with a variety of biological activities such as antifungal, anti-inflammatory, antibacterial, antimicrobial, antiHIV, and anticancer (Dembitsky et al., 2006). Previously, there were many researches about the diyne-structure-related-capability of polyyne, especially ene-diyne, these investigations suggested that enediyne compounds include cardinal triple bonds (diyne) seperated by a double bond (ene) can partitioning DNA via cross linking to several positions, interacting with minor groove or abstracting hydrogen atoms as well as arrest cell cycle, inhibit proteins compulsory for initiation replication stage (Chin et al., 1988 Sugiura et al., 1989 Walker et a... ... nuclei, and should be separated with unsnarl nuclear boundary. Timescale involvedAlong with the proposal, the RAGS system will be entire and approved in week 18. After that, the first st ep is tax deduction of two chemicals in 4 weeks from 16th January to 13th February 2012. Once the chemicals are produced, TK6 cells will be treated in control medium and in medium containing these compounds, separately, and measure LD50 of TK6 cell line, statistic analysis LOEC, NOEC and thereby comparing cytotoxicity of these compounds. This appendage will take around 6 weeks from 13th February to 23rd marching music 2012. Later on, micronucleus assay will be applied to study genotoxicity on TK6 cells via clastogenic behaviour for 7 weeks and finish laboratory practical on initiative June 2012. Finally, writing up and project draft will be realised within 4 weeks.